Protective effects of dioscin against cisplatin-induced nephrotoxicity via the microRNA-34a/sirtuin 1 signalling pathway.

ABSTRACT:

Background and purpose

Dioscin exhibits a range of pharmacological actions but little is known of its effects on cisplatin (CDDP)-induced nephrotoxicity. Here, we have assessed the effects and the possible mechanisms of dioscin against CDDP-induced nephrotoxicity.

Experimental approach

We used an in vivo model of CDDP-induced nephrotoxicity in rats and mice and, in vitro, cultures of NRK-52E and HK-2 cells. The dual luciferase reporter assay was used to demonstrate modulation, by dioscin, of the targeting of sirtuin 1 (Sirt1) by microRNA (miR)-34a. Molecular docking assays were used to analyse the effects of dioscin with Sirt1, Keap1 and NF-κB.

Key results

Dioscin attenuated cell damage in vitro and decreased renal injury in rats and mice, treated with CDDP. In terms

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5513863 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature