The inducible blockage of RNAi reveals a role for polyunsaturated fatty acids in the regulation of dsRNA-endocytic capacity in Bactrocera dorsalis.

ABSTRACT: Exogenous double-stranded RNA (dsRNA) can trigger gene silencing through the RNA interference (RNAi) pathway. Our previous research established that Bactrocera dorsalis can block RNAi after an initial priming of exposure to dsRNA. However, the mechanism underlying this phenomenon is not yet fully understood. Here, we demonstrate that fatty acid biosynthesis and metabolism pathways play important roles in the blockage of RNAi induced by dsRNA priming. The ratio of linoleic acid (LA) to arachidonic acid (AA) was significantly increased in the hemolymph of B. dorsalis following dsRNA priming, and further, the endocytosis of dsRNA into the midgut cells of B. dorsalis was inhibited in these samples. The expression levels of most genes involved in the fatty acid biosynthesis and metabolism pathw

SUBMITTER: Dong X 

PROVIDER: S-EPMC5514080 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature