A Novel Pathogen Capturing Device for Removal and Detection.

ABSTRACT: A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80.3 ± 5.6% of Staphylococcus aureus with a starting concentration of ~10⁷ CFU/mL and 95.4 ± 1.0% of Methicillin-resistant Staphylococcus aureus with starting concentration of ~10⁴ CFU/mL were removed from 5 mL blood in a few hours. This concept was extended to live ra

SUBMITTER: Kim G 

PROVIDER: S-EPMC5514083 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature