MiR-661 promotes tumor invasion and metastasis by directly inhibiting RB1 in non small cell lung cancer.

ABSTRACT:

Background

Aberrant microRNA expression has been implicated in metastasis of cancers. MiR-661 accelerates proliferation and invasion of breast cancer and ovarian cancer, while impedes that of glioma. Its role in non small cell lung cancer (NSCLC) and underlying mechanism are worthy elucidation.

Methods

Expression of miR-661 was measured with real-time PCR in both NSCLC tissues and cell lines. The effects of miR-661 on migration, invasion and metastasis capacity of NSCLC were evaluated using wound healing, transwell assay and animal models. Dual reporter luciferase assay and complementary experiments were performed to validate RB1 as a direct target of miR-661 for participation in the progression of NSCLC.

Results

MiR-661 was upregulated in NSCLC tissues as compared to

SUBMITTER: Liu F 

PROVIDER: S-EPMC5514511 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature