Systematic evaluation of characteristics of the membrane-based fed-batch shake flask.

ABSTRACT:

Background

The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted with a central feed reservoir with an integrated rotating membrane tip for a controlled substrate release. Building on the previously provided proof of principle for this tool, this work extends its application by constructive modifications and improved methodology to ensure reproducible performance.

Results

The previously limited operation window was expanded by a systematic analysis

SUBMITTER: Philip P 

PROVIDER: S-EPMC5514527 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature