Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus.

ABSTRACT:

Background

Goatpox virus (GTPV) and sheeppox virus (SPPV), which belong to the Capripoxvirus (CaPV), are economically important pathogens of small ruminants. Therefore, a sensitive, specific and rapid diagnostic assay for detection of GTPV and SPPV is necessary to accurately and promptly control these diseases.

Methods

Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively.

Results

The sensitivity of both CaPV real-time RPA assay and CaPV RPA LFD assay were 3 × 10² copies per reaction within 20 min at 38 °C. Both assays were highly specific for CaPV, with n

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5514530 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature