Up-regulation of CDK16 by multiple mechanisms in hepatocellular carcinoma promotes tumor progression.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) remains difficult to cure due to lack of effective treatment and the molecular mechanisms are complex and not completely understood. In this study, We investigated the role of CDK16 in tumor progression of HCC.

Methods

We interrogated the expression level of CDK16 by polymerase chain reaction and immunohistochemistry(IHC) and studied its clinical significance. The functional role of CDK16 on HCC was studied via gain and loss of function in vitro and in vivo. Luciferase reporter assay and Chromatin immunoprecipitation(ChIP) assay were performed to investigate the transcriptional and post-transcriptional mechanisms involved in the regulation of CDK16.

Results

CDK16 expression was significantly up-regulated in HCC and higher expr

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5514535 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature