Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples.

ABSTRACT: Our groups have recently developed related approaches for sample preparation for super-resolution imaging within endogenous cellular environments using correlative light and electron microscopy (CLEM). Four distinct techniques for preparing and acquiring super-resolution CLEM data sets for aldehyde-fixed specimens are provided, including Tokuyasu cryosectioning, whole-cell mount, cell unroofing and platinum replication, and resin embedding and sectioning. The choice of the best protocol for a given application depends on a number of criteria that are discussed in detail. Tokuyasu cryosectioning is relatively rapid but is limited to small, delicate specimens. Whole-cell mount has the simplest sample preparation but is restricted to surface structures. Cell unroofing and platinum replication

SUBMITTER: Kopek BG 

PROVIDER: S-EPMC5514615 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature