Experimental evolution reveals an effective avenue to release catabolite repression via mutations in XylR.

ABSTRACT: Microbial production of fuels and chemicals from lignocellulosic biomass provides promising biorenewable alternatives to the conventional petroleum-based products. However, heterogeneous sugar composition of lignocellulosic biomass hinders efficient microbial conversion due to carbon catabolite repression. The most abundant sugar monomers in lignocellulosic biomass materials are glucose and xylose. Although industrial Escherichia coli strains efficiently use glucose, their ability to use xylose is often repressed in the presence of glucose. Here we independently evolved three E. coli strains from the same ancestor to achieve high efficiency for xylose fermentation. Each evolved strain has a point mutation in a transcriptional activator for xylose catabolic operons, either CRP

SUBMITTER: Sievert C 

PROVIDER: S-EPMC5514714 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature