The oxidation state of the cytoplasmic glutathione redox system does not correlate with replicative lifespan in yeast.

ABSTRACT: What is cause and what is consequence of aging and whether reactive oxygen species (ROS) contribute to this phenomenon is debated since more than 50 years. Notwithstanding, little is known about the cellular buffer and redox systems in aging Saccharomyces cerevisiae, which is a model for aging stem cells. Using genetically encoded fluorescent sensors, we measured pH, H₂O₂ levels and the glutathione redox potential compartment-specific in the cytosol of living, replicatively aging yeast cells, growing under fermenting and respiratory conditions until the end of their lifespan. We found that the pH decreases under both conditions at later stages of the replicative lifespan. H₂O₂ levels increase in fermenting cells in the post-replicative sta

SUBMITTER: Knieß RA 

PROVIDER: S-EPMC5515007 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature