Covalent Ras Dimerization on Membrane Surfaces through Photosensitized Oxidation.

ABSTRACT: Ras, a small GTPase found primarily on the inner leaflet of the plasma membrane, is an important signaling node and an attractive target for anticancer therapies. Lateral organization of Ras on cellular membranes has long been a subject of intense research; in particular, whether it forms dimers on membranes as part of its regulatory function has been a point of great interest. Here we report Ras dimer formation on membranes by Type II photosensitization reactions, in which molecular oxygen mediates the radicalization of proteins under typical fluorescence experimental conditions. The presence of Ras dimers on membranes was detected by diffusion-based fluorescence techniques including fluorescence correlation spectroscopy and single particle tracking, and molecular weights of the stable co

SUBMITTER: Chung JK 

PROVIDER: S-EPMC5515073 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature