Proteomic profiling of early degenerative retina of RCS rats.

ABSTRACT:

Aim

To identify the underlying cellular and molecular changes in retinitis pigmentosa (RP).

Methods

Label-free quantification-based proteomics analysis, with its advantages of being more economic and consisting of simpler procedures, has been used with increasing frequency in modern biological research. Dystrophic RCS rats, the first laboratory animal model for the study of RP, possess a similar pathological course as human beings with the diseases. Thus, we employed a comparative proteomics analysis approach for in-depth proteome profiling of retinas from dystrophic RCS rats and non-dystrophic congenic controls through Linear Trap Quadrupole - orbitrap MS/MS, to identify the significant differentially expressed proteins (DEPs). Bioinformatics analyses, including Gene ontolo

SUBMITTER: Zhu ZH 

PROVIDER: S-EPMC5515160 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature