Somatic mutations in leukocytes infiltrating primary breast cancers.

ABSTRACT:

Background

Malignant transformation requires the interaction of cancer cells with their microenvironment, including infiltrating leukocytes. However, somatic mutational studies have focused on alterations in cancer cells, assuming that the microenvironment is genetically normal. Because we hypothesized that this might not be a valid assumption, we performed exome sequencing and targeted sequencing to investigate for the presence of pathogenic mutations in tumor-associated leukocytes in breast cancers.

Methods

We used targeted sequencing and exome sequencing to evaluate the presence of mutations in sorted tumor-infiltrating CD45-positive cells from primary untreated breast cancers. We used high-depth sequencing to determine the presence/absence of the mutations we identified

SUBMITTER: Kleppe M 

PROVIDER: S-EPMC5515194 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature