Mutant selection in the self-incompatible plant radish (Raphanus sativus L. var. sativus) using two-step TILLING.

ABSTRACT: Radish (Raphanus sativus L. var. sativus), a widely cultivated root vegetable crop, possesses a large sink organ (the root), implying that photosynthetic activity in radish can be enhanced by altering both the source and sink capacity of the plant. However, since radish is a self-incompatible plant, improved mutation-breeding strategies are needed for this crop. TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful method used for reverse genetics. In this study, we developed a new TILLING strategy involving a two-step mutant selection process for mutagenized radish plants: the first selection is performed to identify a BC₁M₁ line, that is, progenies of M₁ plants crossed with wild-type, and the second step is performed to identify

SUBMITTER: Kohzuma K 

PROVIDER: S-EPMC5515317 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature