Predicting the protein half-life in tissue from its cellular properties.

ABSTRACT: Protein half-life is an important feature of protein homeostasis (proteostasis). The increasing number of in vivo and in vitro studies using high throughput proteomics provide estimates of the protein half-lives in tissues and cells. However, protein half-lives in cells and tissues are different. Due to the resource requirements for researching tissues, more data is available from cellular studies than tissues. We have designed a multivariate linear model for predicting protein half-life in tissue from its cellular properties. Inputs to the model are cellular half-life, abundance, intrinsically disordered sequences, and transcriptional and translational rates. Before the modeling, we determined substructures in the data using the relative distance from the regression line of the protein ha

SUBMITTER: Rahman M 

PROVIDER: S-EPMC5515413 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature