Visual detection of Brucella in bovine biological samples using DNA-activated gold nanoparticles.

ABSTRACT: Brucellosis is a bacterial disease, which, although affecting cattle primarily, has been associated with human infections, making its detection an important challenge. The existing gold standard diagnosis relies on the culture of bacteria which is a lengthy and costly process, taking up to 45 days. New technologies based on molecular diagnosis have been proposed, either through dip-stick, immunological assays, which have limited specificity, or using nucleic acid tests, which enable to identify the pathogen, but are impractical for use in the field, where most of the reservoir cases are located. Here we demonstrate a new test based on hybridization assays with metal nanoparticles, which, upon detection of a specific pathogen-derived DNA sequence, yield a visual colour change. We characteri

SUBMITTER: Pal D 

PROVIDER: S-EPMC5515423 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature