Phenotypic characterization of circulating tumor cells in the peripheral blood of patients with small cell lung cancer.

ABSTRACT:

Background

To evaluate the phenotypic heterogeneity of circulating tumor cells (CTCs) based on the expression of proliferative, apoptotic and Epithelial-to-Mesenchymal Transmission (EMT) markers during front-line treatment in patients with small cell lung cancer (SCLC) and to evaluate their clinical relevance.

Methods

CTCs from 108 chemotherapy-naïve patients with SCLC were analyzed by double immunofluorescence staining using anti-Ki67, anti-M30, anti-Vimentin along with anti-CKs antibodies. In 83 patients CTCs were also enumerated using the CellSearch.

Results

Sequential samples were available from 76 and 48 patients after one-treatment cycle and on disease progression (PD), respectively, for immunofluorescence and from 50 and 36 patients after one-cycle and on PD, r

SUBMITTER: Messaritakis I 

PROVIDER: S-EPMC5515424 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature