Effects of angular frequency during clinorotation on mesenchymal stem cell morphology and migration.

ABSTRACT:

Aims

To determine the short-term effects of simulated microgravity on mesenchymal stem cell behaviors-as a function of clinorotation speed-using time-lapse microscopy.

Background

Ground-based microgravity simulation can reproduce the apparent effects of weightlessness in spaceflight using clinostats that continuously reorient the gravity vector on a specimen, creating a time-averaged nullification of gravity. In this work, we investigated the effects of clinorotation speed on the morphology, cytoarchitecture, and migration behavior of human mesenchymal stem cells (hMSCs).

Methods

We compared cell responses at clinorotation speeds of 0, 30, 60, and 75 rpm over 8 h in a recently developed lab-on-chip-based clinostat system. Time-lapse light microscopy was used to visual

SUBMITTER: Luna C 

PROVIDER: S-EPMC5515506 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature