The effect of spaceflight on the gravity-sensing auxin gradient of roots: GFP reporter gene microscopy on orbit.

ABSTRACT: Our primary aim was to determine whether gravity has a direct role in establishing the auxin-mediated gravity-sensing system in primary roots. Major plant architectures have long been thought to be guided by gravity, including the directional growth of the primary root via auxin gradients that are then disturbed when roots deviate from the vertical as a gravity sensor. However, experiments on the International Space Station (ISS) now allow physical clarity with regard to any assumptions regarding the role of gravity in establishing fundamental root auxin distributions. We examined the spaceflight green fluorescent protein (GFP)-reporter gene expression in roots of transgenic lines of Arabidopsis thaliana: pDR5r::GFP, pTAA1::TAA1-GFP, pSCR::SCR-GFP to monitor auxin and pARR5::GFP to

SUBMITTER: Ferl RJ 

PROVIDER: S-EPMC5515520 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature