Post-entrapment genome engineering: first exon size does not affect the expression of fusion transcripts generated by gene entrapment.

ABSTRACT: Gene trap mutagenesis in mouse embryonic stem cells has been widely used for genome-wide studies of mammalian gene function. However, while large numbers of genes can be disrupted, individual mutations may suffer from limitations due to the structure and/or placement of targeting vector. To extend the utility of gene trap mutagenesis, replaceable 3' [or poly(A)] gene trap vectors were developed that permit sequences inserted in individual entrapment clones to be engineered by Cre-mediated recombination. 3' traps incorporating different drug resistance genes could be readily exchanged, simply by selecting for the drug-resistance gene of the replacement vector. By substituting different 3' traps, we show that otherwise identical fusion genes containing a large first exon (804 nt) are not exp

SUBMITTER: Osipovich AB 

PROVIDER: S-EPMC551569 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature