A novel fluorescent probe-based flow cytometric assay for mineral-containing nanoparticles in serum.

ABSTRACT: Calciprotein particles, nanoscale aggregates of insoluble mineral and binding proteins, have emerged as potential mediators of phosphate toxicity in patients with Chronic Kidney Disease. Although existing immunochemical methods for their detection have provided compelling data, these approaches are indirect, lack specificity and are subject to a number of other technical and theoretical shortcomings. Here we have developed a rapid homogeneous fluorescent probe-based flow cytometric method for the detection and quantitation of individual mineral-containing nanoparticles in human and animal serum. This method allows the discrimination of membrane-bound from membrane-free particles and different mineral phases (amorphous vs. crystalline). Critically, the method has been optimised for use on a

SUBMITTER: Smith ER 

PROVIDER: S-EPMC5515983 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature