Small RNA mediated repression of subtilisin production in Bacillus licheniformis.

ABSTRACT: The species Bacillus licheniformis includes important strains that are used in industrial production processes. Currently the physiological model used to adapt these processes is based on the closely related model organism B. subtilis. In this study we found that both organisms reveal significant differences in the regulation of subtilisin, their main natural protease and a product of industrial fermentation processes. We identified and characterized a novel antisense sRNA AprAs, which represents an RNA based repressor of apr, the gene encoding for the industrial relevant subtilisin protease. Reduction of the AprAs level leads to an enhanced proteolytic activity and an increase of Apr protein expression in the mutant strain. A vector based complementation of the AprAs deficient mutant conf

SUBMITTER: Hertel R 

PROVIDER: S-EPMC5516005 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature