Selection of reference genes for gene expression studies in human neutrophils by real-time PCR.

ABSTRACT:

Background

Reference genes, which are often referred to housekeeping genes, are frequently used to normalize mRNA levels between different samples. However the expression level of these genes may vary among tissues or cells, and may change under certain circumstances. Thus the selection of reference gene(s) is critical for gene expression studies. For this purpose, 10 commonly used housekeeping genes were investigated in isolated human neutrophils.

Results

Initial screening of the expression pattern demonstrated that 3 of the 10 genes were expressed at very low levels in neutrophils and were excluded from further analysis. The range of expression stability of the other 7 genes was (from most stable to least stable): GNB2L1 (Guanine nucleotide binding protein, beta polypeptid

SUBMITTER: Zhang X 

PROVIDER: S-EPMC551605 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature