Impact of Paracoccin Gene Silencing on Paracoccidioides brasiliensis Virulence.

ABSTRACT: Among the endemic deep mycoses in Latin America, paracoccidioidomycosis (PCM), caused by thermodimorphic fungi of the Paracoccidioides genus, is a major cause of morbidity. Disease development and its manifestations are associated with both host and fungal factors. Concerning the latter, several recent studies have employed the methodology of gene modulation in P. brasiliensis using antisense RNA (AsRNA) and Agrobacterium tumefaciens-mediated transformation (ATMT) to identify proteins that influence fungus virulence. Our previous observations suggested that paracoccin (PCN), a multidomain fungal protein with both lectin and enzymatic activities, may be a potential P. brasiliensis virulence factor. To explore this, we used AsRNA and ATMT methodology to obtain thr

SUBMITTER: Fernandes FF 

PROVIDER: S-EPMC5516250 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature