A small-molecule/cytokine combination enhances hematopoietic stem cell proliferation via inhibition of cell differentiation.

ABSTRACT:

Background

Accumulated evidence supports the potent stimulating effects of multiple small molecules on the expansion of hematopoietic stem cells (HSCs) which are important for the therapy of various hematological disorders. Here, we report a novel, optimized formula, named the SC cocktail, which contains a combination of three such small molecules and four cytokines.

Methods

Small-molecule candidates were individually screened and then combined at their optimal concentration with the presence of cytokines to achieve maximum capacity for stimulating the human CD34⁺ cell expansion ex vivo. The extent of cell expansion and the immunophenotype of expanded cells were assessed through flow cytometry. The functional preservation of HSC stemness was confirmed by additiona

SUBMITTER: Wang L 

PROVIDER: S-EPMC5516306 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature