Reversible bacterial immobilization based on the salt-dependent adhesion of the bacterionanofiber protein AtaA.

ABSTRACT:

Background

Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enzymatic activity, and permits repeated or continuous biocatalyst use. However, conventional immobilization methods have practical limitations, such as limited mass transfer in the inner part of a gel, gel fragility, cell leakage from the support matrix, and adverse effects on cell viability and catalytic activity. We previously showed a new method for bacterial cell immobilization using AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5. This approach is expected to solve the drawbacks of conventional immobilization methods

SUBMITTER: Yoshimoto S 

PROVIDER: S-EPMC5516326 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature