MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis.

ABSTRACT:

Background

Controversy exists regarding which cell types are responsible for autoantigen presentation in the retina during experimental autoimmune uveitis (EAU) development. In this study, we aimed to identify and characterize the retinal resident and infiltrating cells susceptible to express major histocompatibility complex (MHC) class II during EAU.

Methods

EAU was induced in C57BL/6 mice by adoptive transfer of autoreactive lymphocytes from IRBP1-20-immunized animals. MHC class II expression was studied by immunostainings on eye cryosections. For flow cytometry (FC) analysis, retinas were dissected and enzymatically digested into single-cell suspensions. Three MHC class II⁺ retinal cell populations were sorted by FC, and their RNA processed for RNA-Seq.

Resu

SUBMITTER: Lipski DA 

PROVIDER: S-EPMC5516361 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature