Oxysterols synergize with statins by inhibiting SREBP-2 in ovarian cancer cells.

ABSTRACT:

Objective

Determine mechanisms responsible for enhanced statin efficacy in a novel statin combination we name STOX (STatin-OXysterol).

Methods

Ovarian cancer cell lines were treated with combinations of statins and oxysterols. Cell viability was determined by a modified MTT assay. Apoptosis was evaluated by immunoblotting of PARP and DAPI-mediated visualization of apoptotic nuclei. STOX effects on the expression of genes of the mevalonate pathway were assessed by real-time qPCR and immunoblotting. siRNA-mediated gene silencing was used to test the involvement of oxysterol-mediated repression of SREBP-2 in STOX synergy. The impact of statin-mediated inhibition of protein prenylation and on cholesterol homeostasis was evaluated.

Results

Oxysterols dramatically enhance c

SUBMITTER: Casella C 

PROVIDER: S-EPMC5516475 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature