A Ruthenium(II) Complex as a Luminescent Probe for DNA Mismatches and Abasic Sites.

ABSTRACT: [Ru(bpy)₂(BNIQ)]²⁺ (BNIQ = Benzo[c][1,7]naphthyridine-1-isoquinoline), which incorporates the sterically expansive BNIQ ligand, is a highly selective luminescent probe for DNA mismatches and abasic sites, possessing a 500-fold higher binding affinity toward these destabilized regions relative to well-matched base pairs. As a result of this higher binding affinity, the complex exhibits an enhanced steady-state emission in the presence of DNA duplexes containing a single base mismatch or abasic site compared to fully well-matched DNA. Luminescence quenching experiments with Cu(phen)₂²⁺ and [Fe(CN)₆]^3- implicate binding of the complex to a mismatch from the minor groove via metalloinsertion. The emission response of the complex

SUBMITTER: Boynton AN 

PROVIDER: S-EPMC5516787 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature