Rapid reconstitution of antibody responses following transplantation of purified allogeneic hematopoietic stem cells.

ABSTRACT: Allogeneic hematopoietic cell transplantation has broad clinical applications extending from the treatment of malignancies to induction of immunologic tolerance. However, adaptive cellular and humoral immunity frequently remain impaired posttransplantation. Here, recovery of T-dependent and T-independent Ab responses was evaluated in mice transplanted with purified hematopoietic stem cells (HSCs) devoid of the mature immune cells believed to hasten immune recovery. Mixed and full donor chimeras were created by conditioning recipients with sublethal or lethal irradiation, respectively, across different donor/host genetic disparities. By 6 wk posttransplantation, all animals demonstrated robust T-independent Ab responses, and all mixed chimeras and recipients of MHC-matched or haploidentical

SUBMITTER: Linderman JA 

PROVIDER: S-EPMC5516799 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature