Cross-species gene expression analysis identifies a novel set of genes implicated in human insulin sensitivity.

ABSTRACT:

Objective

Insulin resistance (IR) is one of the earliest predictors of type 2 diabetes. However, diagnosis of IR is limited. High fat fed mouse models provide key insights into IR. We hypothesized that early features of IR are associated with persistent changes in gene expression (GE) and endeavored to (a) develop novel methods for improving signal:noise in analysis of human GE using mouse models; (b) identify a GE motif that accurately diagnoses IR in humans; and (c) identify novel biology associated with IR in humans.

Methods

We integrated human muscle GE data with longitudinal mouse GE data and developed an unbiased three-level cross-species analysis platform (single gene, gene set, and networks) to generate a gene expression motif (GEM) indicative of IR. A logistic regre

SUBMITTER: Chaudhuri R 

PROVIDER: S-EPMC5516867 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature