Regulation of amino-acid metabolism controls flux to lipid accumulation in Yarrowia lipolytica.

ABSTRACT: Yarrowia lipolytica is a promising microbial cell factory for the production of lipids to be used as fuels and chemicals, but there are few studies on regulation of its metabolism. Here we performed the first integrated data analysis of Y. lipolytica grown in carbon and nitrogen limited chemostat cultures. We first reconstructed a genome-scale metabolic model and used this for integrative analysis of multilevel omics data. Metabolite profiling and lipidomics was used to quantify the cellular physiology, while regulatory changes were measured using RNAseq. Analysis of the data showed that lipid accumulation in Y. lipolytica does not involve transcriptional regulation of lipid metabolism but is associated with regulation of amino-acid biosynthesis, resulting in redirecti

SUBMITTER: Kerkhoven EJ 

PROVIDER: S-EPMC5516929 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature