Comparative analysis of vaginal microbiota sampling using 16S rRNA gene analysis.

ABSTRACT:

Background

Molecular methods such as next-generation sequencing are actively being employed to characterize the vaginal microbiota in health and disease. Previous studies have focused on characterizing the biological variation in the microbiota, and less is known about how factors related to sampling contribute to the results. Our aim was to investigate the impact of a sampling device and anatomical sampling site on the quantitative and qualitative outcomes relevant for vaginal microbiota research. We sampled 10 Finnish women representing diverse clinical characteristics with flocked swabs, the Evalyn® self-sampling device, sterile plastic spatulas and a cervical brush that were used to collect samples from fornix, vaginal wall and cervix. Samples were compared on DNA and protein y

SUBMITTER: Virtanen S 

PROVIDER: S-EPMC5517051 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature