PH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors.

ABSTRACT: Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient's disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the development of single fluorophore and dual fluorophore ratiometric biosensors based on alginate microspheres for pH and urea analysis in urine samples. A facile method of air driven atomization was used for developing these polymeric fluorophore and enzyme based biosensors. Ratiometric biosensors were developed using layer-by-layer coating of polyelectrolyte conjugated to reference fluorophores. Biosensing studies using these biosensors showed that samples in pathophysiological range can be measured having

SUBMITTER: Chaudhari R 

PROVIDER: S-EPMC5517509 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature