Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate.

ABSTRACT: Fatty acid (FA) metabolism directly influences the functional capabilities of T cells in tumor microenvironments. Thus, developing tools to interrogate FA-uptake by T cell subsets is important for understanding tumor immunosuppression. Herein, we have generated a novel FA-Qdot 605 dye conjugate with superior sensitivity and flexibility to any of the previously commercially available alternatives. For the first time, we demonstrate that this nanoparticle can be used as a specific measure of fatty acid uptake by T cells both in-vitro and in-vivo. Flow cytometric analysis shows that both the location and activation status of T cells determines their FA uptake. Additionally, CD4+ Foxp3+ regulatory T cells (Tregs) uptake FA at a higher rate than effector T cell subsets, supporting the role of F

SUBMITTER: Muroski ME 

PROVIDER: S-EPMC5517517 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature