Impairment of Coronary Endothelial Function by Hypoxia-Reoxygenation Involves TRPC3 Inhibition-mediated K_Ca Channel Dysfunction: Implication in Ischemia-Reperfusion Injury.

ABSTRACT: Despite increasing knowledge of the significance of calcium-activated potassium (K_Ca) and canonical transient receptor potential (TRPC) channels in endothelial physiology, no studies so far have investigated the link between these two distinct types of channels in the control of vascular tone in pathological conditions. We previously demonstrated that hypoxia-reoxygenation (H-R) inhibits endothelial K_Ca and TRPC3 channels in porcine coronary arteries (PCAs). The present study further investigated whether modulation of TRPC3 is involved in H-R-induced K_Ca channel inhibition and associated vasodilatory dysfunction using approaches of wire myography, whole-cell voltage-clamp, and coimmunoprecipitation. Pharmacological inhibition or siRNA silencing of TRPC3 sig

SUBMITTER: Wang XC 

PROVIDER: S-EPMC5517640 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature