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Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data.

ABSTRACT: Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.

SUBMITTER: Lutzmayer S 

PROVIDER: S-EPMC5517642 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data.

Lutzmayer Stefan S   Enugutti Balaji B   Nodine Michael D MD  

Scientific reports 20170719 1

Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used  ...[more]

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