Proteomic and bioinformatics profile of paired human alveolar macrophages and peripheral blood monocytes.

ABSTRACT: Little is known about proteomic differences between pluripotent human peripheral blood monocytes (MN) and their terminally-differentiated pulmonary counterparts, alveolar macrophages (AM). To better characterize these cell populations, we performed a label-free shotgun proteomics assessment of matched AM and MN preparations from eight healthy volunteers. With an FDR of less than 0.45%, we identified 1754 proteins within AM and 1445 from MN. Comparison of the two proteomes revealed that 1239 of the proteins found in AM were shared with MN, whereas 206 proteins were uniquely identified in MN and 515 were unique to AM. Molecular and cellular functions, protein classes, development associations, and membership in physiological systems and canonical pathways were identified among the detected p

SUBMITTER: Tomechko SE 

PROVIDER: S-EPMC5518170 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature