The Munich MIDY Pig Biobank - A unique resource for studying organ crosstalk in diabetes.

ABSTRACT:

Objective

The prevalence of diabetes mellitus and associated complications is steadily increasing. As a resource for studying systemic consequences of chronic insulin insufficiency and hyperglycemia, we established a comprehensive biobank of long-term diabetic INS^C94Y transgenic pigs, a model of mutant INS gene-induced diabetes of youth (MIDY), and of wild-type (WT) littermates.

Methods

Female MIDY pigs (n = 4) were maintained with suboptimal insulin treatment for 2 years, together with female WT littermates (n = 5). Plasma insulin, C-peptide and glucagon levels were regularly determined using specific immunoassays. In addition, clinical chemical, targeted metabolomics, and lipidomics analyses were performed. At age 2 years, all pigs were euthanized,

SUBMITTER: Blutke A 

PROVIDER: S-EPMC5518720 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature