Repletion of branched chain amino acids reverses mTORC1 signaling but not improved metabolism during dietary protein dilution.

ABSTRACT:

Objective

Dietary protein dilution (PD) has been associated with metabolic advantages such as improved glucose homeostasis and increased energy expenditure. This phenotype involves liver-induced release of FGF21 in response to amino acid insufficiency; however, it has remained unclear whether dietary dilution of specific amino acids (AAs) is also required. Circulating branched chain amino acids (BCAAs) are sensitive to protein intake, elevated in the serum of obese humans and mice and thought to promote insulin resistance. We tested whether replenishment of dietary BCAAs to an AA-diluted (AAD) diet is sufficient to reverse the glucoregulatory benefits of dietary PD.

Methods

We conducted AA profiling of serum from healthy humans and lean and high fat-fed or New Zealand obese

SUBMITTER: Maida A 

PROVIDER: S-EPMC5518726 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature