Clinical value of R-spondins in triple-negative and metaplastic breast cancers.

ABSTRACT:

Background

RSPO ligands, activators of the Wnt/β-catenin pathway, are overexpressed in different cancers. The objective of this study was to investigate the role of RSPOs in breast cancer (BC).

Methods

Expression of RSPO and markers of various cancer pathways were measured in breast tumours and cell lines by qRT-PCR. The effect of RSPO on the Wnt/β-catenin pathway activity was determined by luciferase assay, western blotting, and qRT-PCR. The effect of RSPO2 inhibition on proliferation was determined by using RSPO2 siRNAs. The effect of IWR-1, an inhibitor of the Wnt/β-catenin pathway, was examined on the growth of an RSPO2-positive patient-derived xenograft (PDX) model of metaplastic triple-negative BC.

Results

We detected RSPO2 and RSPO4 overexpression levels in BC,

SUBMITTER: Coussy F 

PROVIDER: S-EPMC5518860 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature