Substance P increases liver fibrosis by differential changes in senescence of cholangiocytes and hepatic stellate cells.

ABSTRACT: Substance P (SP) is involved in the proliferation of cholangiocytes in bile duct-ligated (BDL) mice and human cholangiocarcinoma growth by interacting with the neurokinin-1 receptor (NK-1R). To identify whether SP regulates liver fibrosis during cholestasis, wild-type or NK-1R knockout (NK-1R^-/- ) mice that received BDL or sham surgery and multidrug resistance protein 2 knockout (Mdr2^-/- ) mice treated with either an NK-1R antagonist (L-733,060) or saline were used. Additionally, wild-type mice were treated with SP or saline intraperitoneally. In vivo, there was increased expression of tachykinin precursor 1 (coding SP) and NK-1R in both BDL and Mdr2^-/- mice compared to wild-type mice. Expression of tachykinin precursor 1 and NK-1R was significantly higher

SUBMITTER: Wan Y 

PROVIDER: S-EPMC5519428 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature