Origin of Enriched Regulatory T Cells in Patients Receiving Combined Kidney-Bone Marrow Transplantation to Induce Transplantation Tolerance.

ABSTRACT: We examined tolerance mechanisms in patients receiving HLA-mismatched combined kidney-bone marrow transplantation (CKBMT) that led to transient chimerism under a previously published nonmyeloablative conditioning regimen (Immune Tolerance Network study 036). Polychromatic flow cytometry and high-throughput sequencing of T cell receptor-β hypervariable regions of DNA from peripheral blood regulatory T cells (Tregs) and CD4 non-Tregs revealed marked early enrichment of Tregs (CD3⁺ CD4⁺ CD25^high CD127^low Foxp3⁺ ) in blood that resulted from peripheral proliferation (Ki67⁺ ), possibly new thymic emigration (CD31⁺ ), and, in one tolerant subject, conversion from non-Tregs. Among recovering conventional T cells, central

SUBMITTER: Sprangers B 

PROVIDER: S-EPMC5519438 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature