ScaffoldSeq: Software for characterization of directed evolution populations.

ABSTRACT: ScaffoldSeq is software designed for the numerous applications-including directed evolution analysis-in which a user generates a population of DNA sequences encoding for partially diverse proteins with related functions and would like to characterize the single site and pairwise amino acid frequencies across the population. A common scenario for enzyme maturation, antibody screening, and alternative scaffold engineering involves naïve and evolved populations that contain diversified regions, varying in both sequence and length, within a conserved framework. Analyzing the diversified regions of such populations is facilitated by high-throughput sequencing platforms; however, length variability within these regions (e.g., antibody CDRs) encumbers the alignment process. To overcome this chall

SUBMITTER: Woldring DR 

PROVIDER: S-EPMC5519505 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature