⁶⁴Cu-Labeled Gp2 Domain for PET Imaging of Epidermal Growth Factor Receptor.

ABSTRACT: This purpose of this study is to determine the efficacy of a 45-amino acid Gp2 domain, engineered to bind to epidermal growth factor receptor (EGFR), as a positron emission tomography (PET) probe of EGFR in a xenograft mouse model. The EGFR-targeted Gp2 (Gp2-EGFR) and a nonbinding control were site-specifically labeled with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelator. Binding affinity was tested toward human EGFR and mouse EGFR. Biological activity on downstream EGFR signaling was examined in cell culture. DOTA-Gp2 molecules were labeled with ⁶⁴Cu and intravenously injected (0.6-2.3 MBq) into mice bearing EGFR^high (n = 7) and EGFR^low (n = 4) xenografted tumors. PET/computed tomography (CT) images were acquired at 45 min, 2 h, an

SUBMITTER: Kruziki MA 

PROVIDER: S-EPMC5519506 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature