Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β.

ABSTRACT: Protofibrils of the 42 amino acids long amyloid-β peptide are transient pre-fibrillar intermediates in the process of peptide aggregation into amyloid plaques and are thought to play a critical role in the pathology of Alzheimer's disease. Hence, there is a need for research reagents and potential diagnostic reagents for detection and imaging of such aggregates. Here we describe an in vitro selection of Affibody molecules that bind to protofibrils of Aβ₄₂cc, which is a stable engineered mimic of wild type Aβ₄₂ protofibrils. Several binders were identified that bind Aβ₄₂cc protofibrils with low nanomolar affinities, and which also recognize wild type Aβ₄₂ protofibrils. Dimeric head-to-tail fusion proteins with subnanomolar binding affinities, and

SUBMITTER: Wahlberg E 

PROVIDER: S-EPMC5519597 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature