Inhibition of TGF-β signaling supports high proliferative potential of diverse p63⁺ mouse epithelial progenitor cells in vitro.

ABSTRACT: Mouse models have been used to provide primary cells to study physiology and pathogenesis of epithelia. However, highly efficient simple approaches to propagate mouse primary epithelial cells remain challenging. Here, we show that pharmacological inhibition of TGF-β signaling enables long-term expansion of p63⁺ epithelial progenitor cells in low Ca²⁺ media without the need of progenitor cell-purification steps or support by a feeder cell layer. We find that TGF-β signaling is operative in mouse primary keratinocytes in conventional cultures as determined by the nuclear Smad2/3 localization. Accordingly, TGF-β signaling inhibition in crude preparations of mouse epidermis robustly increases proliferative capacity of p63⁺ epidermal progenitor cells, while pres

SUBMITTER: Suzuki D 

PROVIDER: S-EPMC5519764 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature