Excess of a Rassf1-targeting microRNA, miR-193a-3p, perturbs cell division fidelity.

ABSTRACT:

Background

Several microRNA (miRNA) molecules have emerged as important post-transcriptional regulators of tumour suppressor and oncogene expression. Ras association domain family member 1 (RASSF1) is a critical tumour suppressor that controls multiple aspects of cell proliferation such as cell cycle, cell division and apoptosis. The expression of RASSF1 is lost in a variety of cancers due to the promoter hypermethylation.

Methods

miR-193a-3p was identified as a RASSF1-targeting miRNA by a dual screening approach. In cultured human cancer cells, immunoblotting, qRT-PCR, luciferase reporter assays, time-lapse microscopy and immunofluorescence methods were used to study the effects of excess miR-193a-3p on RASSF1 expression and cell division.

Results

Here, we report a n

SUBMITTER: Pruikkonen S 

PROVIDER: S-EPMC5520089 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature