An enhanced bioluminescence-based Annexin V probe for apoptosis detection in vitro and in vivo.

ABSTRACT: The process of controlled cellular death known as apoptosis has an important central role not only in normal homeostatic maintenance of tissues, but also in numerous diseases such as cancer, neurodegenerative, autoimmune, and cardiovascular diseases. As a result, new technologies with the capability to selectively detect apoptotic cells represent a central focus of research for the study of these conditions. We have developed a new biosensor for the detection of apoptotic cells, incorporating the targeted selectivity for apoptotic cells from Annexin V with the sensitivity of bioluminescence signal generation from a serum-stable mutant of Renilla luciferase (RLuc8). Our data presents a complete characterization of the structural and biochemical properties of this new Annexin-Renilla fusion

SUBMITTER: Head T 

PROVIDER: S-EPMC5520691 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature