Enzyme adsorption-induced activity changes: a quantitative study on TiO₂ model agglomerates.

ABSTRACT:

Background

Activity retention upon enzyme adsorption on inorganic nanostructures depends on different system parameters such as structure and composition of the support, composition of the medium as well as enzyme loading. Qualitative and quantitative characterization work, which aims at an elucidation of the microscopic details governing enzymatic activity, requires well-defined model systems.

Results

Vapor phase-grown and thermally processed anatase TiO₂ nanoparticle powders were transformed into aqueous particle dispersions and characterized by dynamic light scattering and laser Doppler electrophoresis. Addition of β-galactosidase (β-gal) to these dispersions leads to complete enzyme adsorption and the generation of β-gal/TiO₂ heteroaggregates. For l

SUBMITTER: Marquez A 

PROVIDER: S-EPMC5521066 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature